Oxidative Stress In Lupus

"Oxidative stress damage in systemic lupus erythematosus "
"Premature onset of atherogenesis"

Detection of Catalase as a major protein target of the lipid
peroxidation product 4-HNE and the lack of its genetic association as
a risk factor in SLE.
D'Souza A, Kurien BT, Rodgers R, Shenoi J, Kurono S, Matsumoto H,
Hensley K, Nath SK, Scofield RH

BMC Med Genet 2008 Jul 7; 9(1):62.

ABSTRACT:
BACKGROUND: Systemic lupus erythematosus (SLE) is a multifactorial
disorder characterized by the presence of autoantibodies. We and
others have implicated free radical mediated peroxidative damage in
the pathogenesis of SLE. Since harmful free radical products are
formed during this oxidative process, including 4-Hydroxy 2-nonenol (4-
HNE) and malondialdehyde (MDA), we hypothesized that specific HNE-
protein adducts would be present in SLE red blood cell (RBC)
membranes. Catalase is located on chromosome 11p13 where linkage
analysis has revealed a marker in the same region of the genome among
families with thrombocytopenia, a clinical manifestation associated
with severe lupus in SLE affected pedigrees. Moreover, SLE afflicts
African-Americans three times more frequently than their European
American counterparts. Hence we investigated the effects of a genetic
polymorphism of catalase on risk and severity of SLE in 48 pedigrees
with African American ancestry.
METHODS: Tryptic digestion followed by matrix assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS)
analysis was used to identify the protein(s) modified by HNE,
following Coomassie staining to visualize the bands on the acrylamide
gels. Genotyping analysis for the Ci T, -262bp polymorphism in the
promoter region of catalase was performed by PCR-RFLP and direct PCR-
sequencing. We used a apedigree disequilibrium testa for the family
based association analysis, implemented in the PDT program to analyze
the genotyping results.
RESULTS: We found two proteins to be HNE-modified, migrating around 80
and 50 kD respectively. Tryptic digestion followed by matrix assisted
laser desorption/ionization time-of-flight mass spectrometry (MALDI-
TOFMS) analysis of the Coomassie stained 80 kD band revealed that the
target of HNE modification was catalase, a protein shown to associate
with RBC membrane proteins. All the test statistics carried out on the
genotyping analysis for the Ci T, -262bp polymorphism in the promoter
region of catalase were non-significant (p>0.05) in our data, which
suggested that this SNP is not associated with SLE.
CONCLUSION: Our results indicate that catalase is one of the proteins
modified due to oxidative stress. However, catalase may not be a
susceptibility gene for SLE. Nonetheless, catalase is oxidatively
modified among SLE patients. This suggests a possible role between
oxidative modification of catalase and its affects on enzymatic
activity in SLE. An oxidatively modified catalase could be one of the
reasons for lower enzymatic activity among SLE subjects, which in turn
could favor the accumulation of deleterious hydrogen peroxide.
Furthermore, HNE-products are potential neoantigens and could be
involved in the pathogenesis of SLE. Decrease in catalase activity
could affect the oxidant-antioxidant balance. Chronic disturbance of
this balance in patients with SLE may work favorably for the premature
onset of atherogenesis with severe vascular effect.

BMC medical genetics [BMC Med Genet]

--------------------------------------------------------------------------------

Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

On Jul 22, 4:05*am, ironjustice <teamtan...@hotmail.com> wrote:On Jul
22, 4:05 am, ironjustice <teamtan...@hotmail.com> wrote:catalase
oxidatively modified <<

"Catalase is oxidatively modified among SLE patients"
"The target of modification was due to oxidative stress"

"Phytic acid and catalase each attenuated A549 cell DNA damage"

Looks like my free bisphosphonate steps up to .. stand in .. again.

Catalase and phytic acid do the **same thing** in the body.
Catalase is **shown** to BE "modified oxidatively" IN those with SLE.
One might think phytic acid would be considered in lupus.
It could replace / standin for that which catalase is incapable of
accomplishing. DUE TO the oxidative modification inflicted upon
catalase ..

That's using logic and reason and science.

Phytic acid is found in your plants.

It is a free bisphosphonate found in your grub ..

http://tinyurl.com/2wuhuk

They / catalase and / or phytic acid do the same thing.

http://tinyurl.com/6qqefj

Original Contributions
Cigarette smoke augments asbestos-induced alveolar epithelial cell
injury: role of free radicals

David W. Kamp, , *, c, Marc J. Greenberger*, c, Jane S. Sbalchierro*,
b, c, Scott E. Preusen*, c and Sigmund A. Weitzmanb, c

* Division of Pulmonary Medicine, Northwestern University Medical
School, Chicago, IL, USA


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Who loves ya. =CAUSE NO ONE WANTS TO BE AROUND THIS CREATURE, LET
ALONE READ THE SHIT SPEWED FORTH BY HIM REPETITIVELY.
Tom= REALLY A HERMAPHRODITIC NEANDERTHAL THAT WILL NOT GO AWAY.

Jesus Was A Vegetarian! JESUS ATE FISH. HE ATE A MEDITERRANEAN DIET.
http://tinyurl.com/2r2nkh (WHY ARE YOU ADVERTISING THAT YOU HAVE A
TINY***, ARE A TINY LITTLE DUMBASS THAT WON'T GO
AWAY!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!)


Man Is A Herbivore! I AM MAN, I AM HUMAN, I EAT MEAT. MOST PEOPLE EAT
MEAT AND ARE NOT HERBIVORES!
http://tinyurl.com/4rq595 (WHY ARE YOU ADVERTISING THAT YOU HAVE A
TINY***, ARE A TINY LITTLE DUMBASS THAT WON'T GO
AWAY!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!)


DEAD PEOPLE WALKING. DEAD PEOPLE CANNOT TALK, WALK, OR EAT,
REGARDLESS
OF THE BULLSHIT SWIMMING AROUND IN THAT FILTHY, SICK, ANNOYING HEAD
OF
YOURS.
http://tinyurl.com/zk9fk (WHY ARE YOU ADVERTISING THAT YOU HAVE A
TINY***, ARE A TINY LITTLE DUMBASS THAT WON'T GO
AWAY!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!)


STAY OFF ANY THREADS.
HOW DO YOU OWN ANYTHING?
STAY OUT OF THE NEWSGROUPS.
QUIT POSTING NONSENSE THAT NO ONE WANTS TO HEAR.
THE ONLY PURPOSE YOU HAVE IN LIFE IS TO ANNOY THOSE ACTUALLY USING
THESE SERVICES FOR THE INTENDED PURPOSE BY POSTING YOUR SHIT.
QUIT.


YOU FIT THE PROFILE OF THE CYBERSTALKER, CYBERBULLY. YOU ARE THE ONE
and ONLY PERSON DOING THAT TO THE REST OF THE NEWSGROUP SUBSCRIBERS.


GO AWAY.


WHY CAN'T YOU UNDERSTAND THAT **YOUR POSTING IS NOT WANTED HERE*****

On Jul 22, 1:12*pm, aka_velcro <Crystalarev...@gmail.com> wrote:snip
<<

Remember do not break crush chew or inject those meds.


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Wow, oxidative stress plays a role in pathological damage. What a
revelation! Whodathunkit Arealshocka

The wonderful thing is that OUR TOM was the one to bring this fact
to the attention of the medical community. Isn't that great? And a
real
medical doctor, Dr Denham Harman, blatantly plagiarized the
information
and made it look like it was his idea.
Ooops, no, wait. He made that observation in 1957. Maybe it was his
idea after all.

On Jul 23, 5:11*am, Chuck <ShorThi...@aol.com> wrote:
> Wow, oxidative stress plays a role in pathological damage. What a
> revelation! Whodathunkit Arealshocka

On Jul 23, 2:11*am, Chuck <ShorThi...@aol.com> wrote:
Wow, oxidative stress plays a role in pathological damage. What a
revelation! Whodathunkit Arealshocka <<

A real shocker to YOU .. mr .. "nutritionist" ..

Chuck .. what did I tell you about my threads .. ?

Take the advice ..

NOBODY wants to hear from a hasbeen .. buddy ..

Can you .. understand .. ?


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

On Jul 23, 3:15*am, Michael B <baugh...@bellsouth.net> wrote: snip <<

Lefty - you were told to stay off of my threads ..


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

"Lefty - you were told to stay off of my threads .."

But it is not your thread. get over it, once posted it is open season.

Jesus ate a mediterranean diet.

On Jul 23, 7:36�am, ironjustice <teamtan...@hotmail.com> wrote:

> Chuck .. what did I tell you about my threads .. ?

"Your" threads? Ever have the inflammatory / oxdation levels of your
brain tissue tested?

>
> NOBODY wants to hear from a hasbeen .. buddy ..
>
> Can you .. understand .. ?

While you cut and paste all-day, I'm doing real writing, research, and
conference presentations on the subject. You've probably cut and
pasted some of my work.
Get some help, guy. Seriously.
CB

On Jul 23, 6:05*am, Chuck <ShorThi...@aol.com> wrote:Get some help,
guy. Seriously. <<

What part .. OF .. stay off my threads don't ya understand .. chuck ..
you pathetic fkg hasbeen .. ?

What .. part ..


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

On Jul 23, 5:43*am, ferro...@ironcity.com wrotence posted it is open
season.
<<

And who congregates .. ?

Lefties and .. loonies ..

Lefties .. and .. loonies ..

LEFFFTIES .. and .. LOOOONIES ..

Now we got .. lefty .. evidentally / admittedly .. and we got you ..
evidentally .. a .. what .. ?

Sooo .. evidentally .. I should .. rest .. my .. case.


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

> "Lefty - you were told to stay off of my threads .."
>
> But it is not your thread. *get over it, once posted it is open season.
>
> Jesus ate a mediterranean diet.

ironjustice wrote:
>
> On Jul 23, 5:43 am, ferro...@ironcity.com wrotence posted it is open
> season.
> <<
>
> And who congregates .. ?

the faithful?
>
> Lefties and .. loonies ..

progressives and moon worshipers?

>
> Lefties .. and .. loonies ..

ibid

>
> LEFFFTIES .. and .. LOOOONIES ..

copy/paste/capitalize

>
> Now we got .. lefty .. evidentally / admittedly .. and we got you ..

babe


> evidentally .. a .. what .. ?

ok folks - no one tell him - make him continue to guess

>
> Sooo .. evidentally

ob quirk! nothing written so far in this tommy missive to have
'evidence' about


>.. I should .. rest .. my .. case.

should you?

indubitably!

query?

is that the case that you store all those reports of real scientists and
researchers in?

you know, the ones you claim are a result of 'your' work?

the work that earned you the right to a nobel prize?


>
> Who loves ya.
> Tom
>
> Jesus Was A Vegetarian!
> http://tinyurl.com/2r2nkh
>
> Man Is A Herbivore!
> http://tinyurl.com/4rq595
>
> DEAD PEOPLE WALKING
> http://tinyurl.com/zk9fk
>
> > "Lefty - you were told to stay off of my threads .."
> >
> > But it is not your thread. get over it, once posted it is open season.
> >
> > Jesus ate a mediterranean diet.

> Get over it, once posted it is open season.

"And who congregates .. ?"

Who ever wants to for any reason whatsoever or even by pure chance. It's
the nature of news groups.

It's like a conversation in a room and anyone can add their bit. self
serving agenda driven monologues arn't taken as good manners and the
person is soon told to mind their manners.

And when they take ... a ... hissy ... fit: well it ain't a pretty
picture.

Jesus ate a mediterranean diet.

<ferrous@ironcity.com> wrote in message news:g68hnb$75p$1@news.tambov.ru...
>> Get over it, once posted it is open season.
>
> "And who congregates .. ?"
>
> Who ever wants to for any reason whatsoever or even by pure chance. It's
> the nature of news groups.
>
> It's like a conversation in a room and anyone can add their bit. self
> serving agenda driven monologues arn't taken as good manners and the
> person is soon told to mind their manners.
>
> And when they take ... a ... hissy ... fit: well it ain't a pretty
> picture.
>
> Jesus ate a mediterranean diet.

Its soooo easy to click up on newsgroups and back space alt.support.
arthritis out of this stupid conversation which has nothing to do with the
support for people with arthritis.
I for one am so tired of reading peoples responses to someone that has
mental problems. Does it prove that anyone is smarter or more well
adjusted to this world in talking to a troll that can not accept anyone's
facts or opinion???????
Why prove that you are so ignorant to think your words on the internet
can change or deal with the mental working of a troll,,,, a person who can
only make themselves important by the attention they get in being rebuffed
or proven wrong or argued with on a stupid point of medical information
right or wrong....... Please leave alt.support.arthritis out of your
mental weakness and ramblings of wasted conversation with a person who will
not accept what you have to say and is a complete waste of everyone's time
and effort.... Please accept what really is and shut the Hell up or leave
ASA out of what is being said.

Harv

"Harvey R. Stone" <hrstone@swbell.net> wrote in message
news:dEQhk.6520$cn7.985@flpi145.ffdc.sbc.com...
>
> <ferrous@ironcity.com> wrote in message
> news:g68hnb$75p$1@news.tambov.ru...
>>> Get over it, once posted it is open season.
>>
>> "And who congregates .. ?"
>>
>> Who ever wants to for any reason whatsoever or even by pure chance.
>> It's
>> the nature of news groups.
>>
>> It's like a conversation in a room and anyone can add their bit.
>> self
>> serving agenda driven monologues arn't taken as good manners and
>> the
>> person is soon told to mind their manners.
>>
>> And when they take ... a ... hissy ... fit: well it ain't a pretty
>> picture.
>>
>> Jesus ate a mediterranean diet.
>
> Its soooo easy to click up on newsgroups and back space alt.support.
> arthritis out of this stupid conversation which has nothing to do
> with the support for people with arthritis.

Once in a while you might want to take that advice Harv

On Jul 23, 3:56*pm, "Paul T. Holland" <pholl...@bellatlantic.net>
wrote: long winded whack <<

http://kook.us/holland.htm


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk


> ironjustice wrote:
>
> > On Jul 23, 5:43 am, ferro...@ironcity.com wrotence posted it is open
> > season.
> > <<
>
> > And who congregates .. ?
>
> the faithful?
>
>
>
> > Lefties and .. loonies ..
>
> progressives and moon worshipers?
>
>
>
> > Lefties .. and .. loonies ..
>
> ibid
>
>
>
> > LEFFFTIES .. and .. LOOOONIES ..
>
> copy/paste/capitalize
>
>
>
> > Now we got .. lefty .. evidentally / admittedly .. and we got you ..
>
> babe
>
> > evidentally .. a .. what .. ?
>
> ok folks - no one tell him - make him continue to guess
>
>
>
> > Sooo .. evidentally
>
> ob quirk! nothing written so far in this tommy missive to have
> 'evidence' about
>
> >.. I should .. rest .. my .. case.
>
> should you?
>
> indubitably!
>
> query?
>
> is that the case that you store all those reports of real scientists and
> researchers in?
>
> you know, the ones you claim are a result of 'your' work?
>
> the work that earned you the right to a nobel prize?
>
>
>
>
>
> > Who loves ya.
> > Tom
>
> > Jesus Was A Vegetarian!
> >http://tinyurl.com/2r2nkh
>
> > Man Is A Herbivore!
> >http://tinyurl.com/4rq595
>
> > DEAD PEOPLE WALKING
> >http://tinyurl.com/zk9fk
>
> > > "Lefty - you were told to stay off of my threads .."
>
> > > But it is not your thread. *get over it, once posted it is open season.
>
> > > Jesus ate a mediterranean diet.- Hide quoted text -
>
> - Show quoted text -

On Jul 23, 6:12*pm, "Harvey R. Stone" <hrst...@swbell.net> wrote: snip
<<

You were told to stay off of my threads ..
Take .. lefty and the rest of the loons .. with ya .. OFF this thread
which has nothing to do with .. arthritis ..

Lupus .. is .. arthritis .. not like the .. herpes .. you .. have ..

One might think with a disease like .. herpes .. you might be .. un-
hygenic ..

How about it .. Harv .. are you .. grubby ..

Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

You are about as capable of listening as you
> <ferr...@ironcity.com> wrote in messagenews:g68hnb$75p$1@news.tambov.ru....
> >> Get over it, once posted it is open season.
>
> > "And who congregates .. ?"
>
> > Who ever wants to for any reason whatsoever or even by pure chance. *It's
> > the nature of news groups.
>
> > It's like a conversation in a room and anyone can add their bit. *self
> > serving agenda driven monologues arn't taken as good manners and the
> > person is soon told to mind their manners.
>
> > And when they take ... a ... hissy ... fit: well it ain't a pretty
> > picture.
>
> > Jesus ate a mediterranean diet.
>
> Its soooo easy to click up on newsgroups and back space alt.support.
> arthritis out of this stupid conversation which has nothing to do with the
> support for people with arthritis.
> * * I for one am so tired of reading peoples responses to someone that has
> mental problems. * *Does it prove that anyone is smarter or more well
> adjusted to this world in talking to a troll that can not accept anyone's
> facts or opinion???????
> * * Why prove that you are so ignorant to think your words on the internet
> can change or deal with the mental working of a troll,,,, a person who can
> only make themselves important by the attention they get in being rebuffed
> or proven wrong or argued with on a stupid point of medical information
> right or wrong....... * Please leave alt.support.arthritis out of your
> mental weakness and ramblings of wasted conversation with a person who will
> not accept what you have to say and is a complete waste of everyone's time
> and effort.... * Please accept what really is and shut the Hell up or leave
> ASA out of what is being said.
>
> Harv- Hide quoted text -
>
> - Show quoted text -

"Jofirey" <jofirey@sbcglobal.net> wrote in message
news:G0Rhk.18369$Ri.17557@flpi146.ffdc.sbc.com...
>
> "Harvey R. Stone" <hrstone@swbell.net> wrote in message
> news:dEQhk.6520$cn7.985@flpi145.ffdc.sbc.com...
>>
>> <ferrous@ironcity.com> wrote in message
>> news:g68hnb$75p$1@news.tambov.ru...
>>>> Get over it, once posted it is open season.
>>>
>>> "And who congregates .. ?"
>>>
>>> Who ever wants to for any reason whatsoever or even by pure chance. It's
>>> the nature of news groups.
>>>
>>> It's like a conversation in a room and anyone can add their bit. self
>>> serving agenda driven monologues arn't taken as good manners and the
>>> person is soon told to mind their manners.
>>>
>>> And when they take ... a ... hissy ... fit: well it ain't a pretty
>>> picture.
>>>
>>> Jesus ate a mediterranean diet.
>>
>> Its soooo easy to click up on newsgroups and back space alt.support.
>> arthritis out of this stupid conversation which has nothing to do with
>> the support for people with arthritis.
>
> Once in a while you might want to take that advice Harv
>

>> Its soooo easy to click up on newsgroups and back space alt.support.
>> arthritis out of this stupid conversation which has nothing to do with
>> the support for people with arthritis.
>
> Once in a while you might want to take that advice Harv
>
Are you a supporter of conversations with Rusty in ASA Jo? Do you enjoy
the abuse he deals to people that point out his mistakes or outdated
information? Whats on your mind,,,, Jo? I see that my post irritated you
about leaving it out of ASA. Do you want to explain it to me so that I
understand it better?
Harv

"ironjustice" <teamtanner@hotmail.com> wrote in message
news:012ca783-3744-4dba-942a-e704c31f23b7@r66g2000hsg.googlegroups.com...
On Jul 23, 6:12 pm, "Harvey R. Stone" <hrst...@swbell.net> wrote: snip
<<

You were told to stay off of my threads ..
Take .. lefty and the rest of the loons .. with ya .. OFF this thread
which has nothing to do with .. arthritis ..

Lupus .. is .. arthritis .. not like the .. herpes .. you .. have ..

One might think with a disease like .. herpes .. you might be .. un-
hygenic ..

How about it .. Harv .. are you .. grubby ..

Who loves ya.
Tom

Yes,,,, Lupus is a very serious disease that can kill a person and far to
serious to be handled by anyone but a Rheumatologist. Your snips of
information when misunderstood could kill a person or add to a persons
condition. I realize that you could never accept responsibility for what
you post on the internet.
God can love a twisted mind such as yours and I do not seem to be able to
even put up with you without irritation which is my failing.
Harv

"Harvey R. Stone" <hrstone@swbell.net> wrote in message
news:%vShk.33879$ZE5.26457@nlpi061.nbdc.sbc.com...
>
>
>
>>> Its soooo easy to click up on newsgroups and back space
>>> alt.support. arthritis out of this stupid conversation which has
>>> nothing to do with the support for people with arthritis.
>>
>> Once in a while you might want to take that advice Harv
>>
> Are you a supporter of conversations with Rusty in ASA Jo? Do you
> enjoy the abuse he deals to people that point out his mistakes or
> outdated information? Whats on your mind,,,, Jo? I see that my
> post irritated you about leaving it out of ASA. Do you want to
> explain it to me so that I understand it better?
> Harv
>
>
Only that if you expect everyone else to leave it out of ASA, you
might want to leave it out of ASA as well.

Jo

On Jul 23, 8:28*pm, "Harvey R. Stone" <hrst...@swbell.net> wrote:
shtfilledskinbag <<
What part of this did you not understand .. ?

You were told to stay off of my threads ..


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Answering for "Harvey", it's the "my threads" part that I don't
understand.
I don't understand how one person, even if only possessing the
intellectual capacity of a pomegranate, would manage to hold
the notion of possessing ownership of a thread. I just find it
amazing, amusing, asinine, and appalling. And that's just
some of the "a" words, you don't want me to tell you some
of the rest of the alphabet.

On Jul 24, 12:00*am, ironjustice <teamtan...@hotmail.com> wrote:
> On Jul 23, 8:28*pm, "Harvey R. Stone" <hrst...@swbell.net> wrote:
> shtfilledskinbag <<
> What part of this did you not understand .. ?
>
> You were told to stay off of my threads ..
>
> Who loves ya.
> Tom
>
> Jesus Was A Vegetarian!http://tinyurl.com/2r2nkh
>
> Man Is A Herbivore!http://tinyurl.com/4rq595
>
> DEAD PEOPLE WALKINGhttp://tinyurl.com/zk9fk

On Jul 24, 3:25*am, Michael B <baugh...@bellsouth.net> wrote: lefty <<

Lefty .. you were told to stay off of my threads ..


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

"Oxidative stress damage in systemic lupus erythematosus "
"Premature onset of atherogenesis"

Detection of Catalase as a major protein target of the lipid
peroxidation product 4-HNE and the lack of its genetic association as
a risk factor in SLE.
D'Souza A, Kurien BT, Rodgers R, Shenoi J, Kurono S, Matsumoto H,
Hensley K, Nath SK, Scofield RH


BMC Med Genet 2008 Jul 7; 9(1):62.


ABSTRACT:
BACKGROUND: Systemic lupus erythematosus (SLE) is a multifactorial
disorder characterized by the presence of autoantibodies. We and
others have implicated free radical mediated peroxidative damage in
the pathogenesis of SLE. Since harmful free radical products are
formed during this oxidative process, including 4-Hydroxy 2-nonenol
(4-
HNE) and malondialdehyde (MDA), we hypothesized that specific HNE-
protein adducts would be present in SLE red blood cell (RBC)
membranes. Catalase is located on chromosome 11p13 where linkage
analysis has revealed a marker in the same region of the genome among
families with thrombocytopenia, a clinical manifestation associated
with severe lupus in SLE affected pedigrees. Moreover, SLE afflicts
African-Americans three times more frequently than their European
American counterparts. Hence we investigated the effects of a genetic
polymorphism of catalase on risk and severity of SLE in 48 pedigrees
with African American ancestry.
METHODS: Tryptic digestion followed by matrix assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS)
analysis was used to identify the protein(s) modified by HNE,
following Coomassie staining to visualize the bands on the acrylamide
gels. Genotyping analysis for the Ci T, -262bp polymorphism in the
promoter region of catalase was performed by PCR-RFLP and direct PCR-
sequencing. We used a apedigree disequilibrium testa for the family
based association analysis, implemented in the PDT program to analyze
the genotyping results.
RESULTS: We found two proteins to be HNE-modified, migrating around
80
and 50 kD respectively. Tryptic digestion followed by matrix assisted
laser desorption/ionization time-of-flight mass spectrometry (MALDI-
TOFMS) analysis of the Coomassie stained 80 kD band revealed that the
target of HNE modification was catalase, a protein shown to associate
with RBC membrane proteins. All the test statistics carried out on
the
genotyping analysis for the Ci T, -262bp polymorphism in the promoter
region of catalase were non-significant (p>0.05) in our data, which
suggested that this SNP is not associated with SLE.
CONCLUSION: Our results indicate that catalase is one of the proteins
modified due to oxidative stress. However, catalase may not be a
susceptibility gene for SLE. Nonetheless, catalase is oxidatively
modified among SLE patients. This suggests a possible role between
oxidative modification of catalase and its affects on enzymatic
activity in SLE. An oxidatively modified catalase could be one of the
reasons for lower enzymatic activity among SLE subjects, which in
turn
could favor the accumulation of deleterious hydrogen peroxide.
Furthermore, HNE-products are potential neoantigens and could be
involved in the pathogenesis of SLE. Decrease in catalase activity
could affect the oxidant-antioxidant balance. Chronic disturbance of
this balance in patients with SLE may work favorably for the
premature
onset of atherogenesis with severe vascular effect.


BMC medical genetics [BMC Med Genet]


---------------------------------------------------------------------------*-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Aaah yes,,,, so true and thanks.
Harv

>>
> Only that if you expect everyone else to leave it out of ASA, you might
> want to leave it out of ASA as well.
>
> Jo
>

On Wed, 23 Jul 2008 22:18:17 -0500, "Harvey R. Stone"
<hrstone@swbell.net> wrote:

>Are you a supporter of conversations with Rusty in ASA Jo? Do you enjoy
>the abuse he deals to people that point out his mistakes or outdated
>information? Whats on your mind,,,, Jo? I see that my post irritated you
>about leaving it out of ASA. Do you want to explain it to me so that I
>understand it better?

Harvey, why are you such an angry man?

"Lefty .. you were told to stay off of my threads .."

Lefty, you are directed to be on "his threads".

Jesus ate a mediterranean diet.

On Jul 24, 12:12*pm, "Paul T. Holland" <pholl...@bellatlantic.net>
wrote: long windeded whack <<

http://kook.us/holland.htm

"Oxidative stress damage in systemic lupus erythematosus "
"Premature onset of atherogenesis"

Detection of Catalase as a major protein target of the lipid
peroxidation product 4-HNE and the lack of its genetic association as
a risk factor in SLE.
D'Souza A, Kurien BT, Rodgers R, Shenoi J, Kurono S, Matsumoto H,
Hensley K, Nath SK, Scofield RH


BMC Med Genet 2008 Jul 7; 9(1):62.


ABSTRACT:
BACKGROUND: Systemic lupus erythematosus (SLE) is a multifactorial
disorder characterized by the presence of autoantibodies. We and
others have implicated free radical mediated peroxidative damage in
the pathogenesis of SLE. Since harmful free radical products are
formed during this oxidative process, including 4-Hydroxy 2-nonenol
(4-
HNE) and malondialdehyde (MDA), we hypothesized that specific HNE-
protein adducts would be present in SLE red blood cell (RBC)
membranes. Catalase is located on chromosome 11p13 where linkage
analysis has revealed a marker in the same region of the genome among
families with thrombocytopenia, a clinical manifestation associated
with severe lupus in SLE affected pedigrees. Moreover, SLE afflicts
African-Americans three times more frequently than their European
American counterparts. Hence we investigated the effects of a genetic
polymorphism of catalase on risk and severity of SLE in 48 pedigrees
with African American ancestry.
METHODS: Tryptic digestion followed by matrix assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS)
analysis was used to identify the protein(s) modified by HNE,
following Coomassie staining to visualize the bands on the acrylamide
gels. Genotyping analysis for the Ci T, -262bp polymorphism in the
promoter region of catalase was performed by PCR-RFLP and direct PCR-
sequencing. We used a apedigree disequilibrium testa for the family
based association analysis, implemented in the PDT program to analyze
the genotyping results.
RESULTS: We found two proteins to be HNE-modified, migrating around
80
and 50 kD respectively. Tryptic digestion followed by matrix assisted
laser desorption/ionization time-of-flight mass spectrometry (MALDI-
TOFMS) analysis of the Coomassie stained 80 kD band revealed that the
target of HNE modification was catalase, a protein shown to associate
with RBC membrane proteins. All the test statistics carried out on
the
genotyping analysis for the Ci T, -262bp polymorphism in the promoter
region of catalase were non-significant (p>0.05) in our data, which
suggested that this SNP is not associated with SLE.
CONCLUSION: Our results indicate that catalase is one of the proteins
modified due to oxidative stress. However, catalase may not be a
susceptibility gene for SLE. Nonetheless, catalase is oxidatively
modified among SLE patients. This suggests a possible role between
oxidative modification of catalase and its affects on enzymatic
activity in SLE. An oxidatively modified catalase could be one of the
reasons for lower enzymatic activity among SLE subjects, which in
turn
could favor the accumulation of deleterious hydrogen peroxide.
Furthermore, HNE-products are potential neoantigens and could be
involved in the pathogenesis of SLE. Decrease in catalase activity
could affect the oxidant-antioxidant balance. Chronic disturbance of
this balance in patients with SLE may work favorably for the
premature
onset of atherogenesis with severe vascular effect.


BMC medical genetics [BMC Med Genet]


---------------------------------------------------------------------------**-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

"Oxidative stress damage in systemic lupus erythematosus "
"Premature onset of atherogenesis"

Detection of Catalase as a major protein target of the lipid
peroxidation product 4-HNE and the lack of its genetic association as
a risk factor in SLE.
D'Souza A, Kurien BT, Rodgers R, Shenoi J, Kurono S, Matsumoto H,
Hensley K, Nath SK, Scofield RH


BMC Med Genet 2008 Jul 7; 9(1):62.


ABSTRACT:
BACKGROUND: Systemic lupus erythematosus (SLE) is a multifactorial
disorder characterized by the presence of autoantibodies. We and
others have implicated free radical mediated peroxidative damage in
the pathogenesis of SLE. Since harmful free radical products are
formed during this oxidative process, including 4-Hydroxy 2-nonenol
(4-
HNE) and malondialdehyde (MDA), we hypothesized that specific HNE-
protein adducts would be present in SLE red blood cell (RBC)
membranes. Catalase is located on chromosome 11p13 where linkage
analysis has revealed a marker in the same region of the genome among
families with thrombocytopenia, a clinical manifestation associated
with severe lupus in SLE affected pedigrees. Moreover, SLE afflicts
African-Americans three times more frequently than their European
American counterparts. Hence we investigated the effects of a genetic
polymorphism of catalase on risk and severity of SLE in 48 pedigrees
with African American ancestry.
METHODS: Tryptic digestion followed by matrix assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS)
analysis was used to identify the protein(s) modified by HNE,
following Coomassie staining to visualize the bands on the acrylamide
gels. Genotyping analysis for the Ci T, -262bp polymorphism in the
promoter region of catalase was performed by PCR-RFLP and direct PCR-
sequencing. We used a apedigree disequilibrium testa for the family
based association analysis, implemented in the PDT program to analyze
the genotyping results.
RESULTS: We found two proteins to be HNE-modified, migrating around
80
and 50 kD respectively. Tryptic digestion followed by matrix assisted
laser desorption/ionization time-of-flight mass spectrometry (MALDI-
TOFMS) analysis of the Coomassie stained 80 kD band revealed that the
target of HNE modification was catalase, a protein shown to associate
with RBC membrane proteins. All the test statistics carried out on
the
genotyping analysis for the Ci T, -262bp polymorphism in the promoter
region of catalase were non-significant (p>0.05) in our data, which
suggested that this SNP is not associated with SLE.
CONCLUSION: Our results indicate that catalase is one of the proteins
modified due to oxidative stress. However, catalase may not be a
susceptibility gene for SLE. Nonetheless, catalase is oxidatively
modified among SLE patients. This suggests a possible role between
oxidative modification of catalase and its affects on enzymatic
activity in SLE. An oxidatively modified catalase could be one of the
reasons for lower enzymatic activity among SLE subjects, which in
turn
could favor the accumulation of deleterious hydrogen peroxide.
Furthermore, HNE-products are potential neoantigens and could be
involved in the pathogenesis of SLE. Decrease in catalase activity
could affect the oxidant-antioxidant balance. Chronic disturbance of
this balance in patients with SLE may work favorably for the
premature
onset of atherogenesis with severe vascular effect.


BMC medical genetics [BMC Med Genet]


---------------------------------------------------------------------------**-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

"Joan Carter" <spamfree@sentex.ca> wrote in message
news:tt6h849o54iqhejafpckv1helq40vja18i@4ax.com...
> On Wed, 23 Jul 2008 22:18:17 -0500, "Harvey R. Stone"
> <hrstone@swbell.net> wrote:
>
>>Are you a supporter of conversations with Rusty in ASA Jo? Do you enjoy
>>the abuse he deals to people that point out his mistakes or outdated
>>information? Whats on your mind,,,, Jo? I see that my post irritated
>>you
>>about leaving it out of ASA. Do you want to explain it to me so that I
>>understand it better?
>
> Harvey, why are you such an angry man?

Why do you play with trolls?