Catalytic Iron In Asbestos

Characteristics and modifying factors of asbestos-induced oxidative
DNA damage.
Jiang L, Nagai H, Ohara H, Hara S, Tachibana M, Hirano S, Shinohara Y,
Kohyama N, Akatsuka S, Toyokuni S
Cancer Sci 2008 Sep 4.

Respiratory exposure to asbestos has been linked with mesothelioma in
humans.
However, its carcinogenic mechanism is still unclear.
Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches.
Electron spin resonance analyses revealed that crocidolite and amosite
containing high amounts of iron, but not chrysotile, catalyzed
hydroxyl radical generation in the presence of H(2)O(2), which was
enhanced by an iron chelator, nitrilotriacetic acid, and suppressed by
desferal.
Natural iron chelators, such as citrate, adenosine 5'-triphosphate and
guanosine 5'-triphosphate, did not inhibit this reaction. Second, we
used time-lapse video microscopy to evaluate how cells cope with
asbestos fibers.
RAW264.7 cells, MeT-5 A and HeLa cells engulfed asbestos fibers, which
reached not only cytoplasm but also the nucleus. Third, we utilized
supercoiled plasmid DNA to evaluate the ability of each asbestos to
induce DNA double strand breaks (DSB). Crocidolite and amosite, but
not chrysotile, induced DNA DSB in the presence of iron chelators.
We cloned the fragments to identify break sites. DSB occurred
preferentially within repeat sequences and between two G:C sequences.
Finally, i.p. administration of each asbestos to rats induced not only
formation of nuclear 8-hydroxy-2'-deoxyguanosine in the mesothelia,
spleen, liver and kidney but also significant iron deposits in the
spleen.
Together with the established carcinogenicity of i.p. chrysotile, our
data suggest that asbestos-associated catalytic iron, whether
constitutional or induced by other mechanisms, plays an important role
in asbestos-induced carcinogenesis and that chemoprevention may be
possible through targeting the catalytic iron.
Cancer science [Cancer Sci]
--------------------------------------------------------------------------------

Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Please do explain in your own words the meaning of the following from
the abstract:

"Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches. Electron spin resonance analyses revealed that
crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction."

Are you sure you want to post this in support of the iron causes all
disease because people eat meat idea?

On Sep 10, 11:32*am, anonym...@nowhere.you.know wrote:
Are you sure you want to post this in support of the iron causes all
>disease because people eat meat idea? <<

Iron chelators prevent the cancer causing effects of asbestos ..

Now you may have a different understanding .. ?

Explain to everyone .. YOUR .. understanding OF .. "iron chelators
prevent the cancer causing effects of asbestos .." ..

Make it short ..


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Please do explain in your own words the meaning of the following from
the abstract:

"Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches. Electron spin resonance analyses revealed that
crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction."

Are you sure you want to post this in support of the iron causes all
disease because people eat meat idea?

Iron chelators prevent the cancer causing effects of asbestos ..
"
Now you may have a different understanding .. ?"

No, just what it says, read this part again:

"crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction.""

Note in particular what caused a reaction to be "enhanced" and what
caused no reaction difference. Were they the same kind of thing and
does it contridict your belief above?

On Sep 10, 5:14*pm, anonym...@nowhere.you.know wrote:
Please do explain in your own words the meaning of the following from
the abstract: <<

I told you to answer a question ..

You are just as useless as I told you ..

Let me quote .. "suppressed by desferal" ..

Desferal is an iron chelator ..

Iron chelators prevent the cancer causing effects of asbestos ..

Now you may have a different understanding .. ?

Explain to everyone .. YOUR .. understanding OF .. "desferal
prevents the cancer causing effects of asbestos .." ..

Make it short ..

Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Are you sure you want to post this in support of the iron causes all
disease because people eat meat idea?

Iron chelators prevent the cancer causing effects of asbestos ..
"
Now you may have a different understanding .. ?"

No, just what it says, read this part again:

"crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction.""

Note in particular what caused a reaction to be "enhanced" and what
caused no reaction difference. Were they the same kind of thing and
does it contridict your belief above?

Let me quote .. "suppressed by desferal" ..

Desferal is an iron chelator ..

Let me quoate, one chelator "enhances" the bad reaction and 4 others
have no effect. The one that did have a good outcome was not only
because it was a chelator of iron because another increased the bad and
others did nothing. Note also it only happened in the presence of a
chemical. Please tell us what that chemical is. Could that chemical be
the source of problems and could the one chelator that worked act on it
and not the iron because one iron chelator made it worse and others did
nothing?

On Sep 11, 5:53*am, anonym...@nowhere.you.know wrote: snip <<

Desferal prevents the cancer causing effects of asbestos ..


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Are you sure you want to post this in support of the iron causes all
disease because people eat meat idea?

Iron chelators prevent the cancer causing effects of asbestos ..
"
Now you may have a different understanding .. ?"

No, just what it says, read this part again:

"crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction.""

Note in particular what caused a reaction to be "enhanced" and what
caused no reaction difference. Were they the same kind of thing and
does it contridict your belief above?

Let me quote .. "suppressed by desferal" ..

Desferal is an iron chelator ..

Let me quoate, one chelator "enhances" the bad reaction and 4 others
have no effect. The one that did have a good outcome was not only
because it was a chelator of iron because another increased the bad and
others did nothing. Note also it only happened in the presence of a
chemical. Please tell us what that chemical is. Could that chemical be
the source of problems and could the one chelator that worked act on it
and not the iron because one iron chelator made it worse and others did
nothing?

Characteristics and modifying factors of asbestos-induced oxidative
DNA damage.
Jiang L, Nagai H, Ohara H, Hara S, Tachibana M, Hirano S, Shinohara
Y,
Kohyama N, Akatsuka S, Toyokuni S
Cancer Sci 2008 Sep 4.

Respiratory exposure to asbestos has been linked with mesothelioma in
humans.
However, its carcinogenic mechanism is still unclear.
Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches.
Electron spin resonance analyses revealed that crocidolite and
amosite
containing high amounts of iron, but not chrysotile, catalyzed
hydroxyl radical generation in the presence of H(2)O(2), which was
enhanced by an iron chelator, nitrilotriacetic acid, and suppressed
by
desferal.
Natural iron chelators, such as citrate, adenosine 5'-triphosphate
and
guanosine 5'-triphosphate, did not inhibit this reaction. Second, we
used time-lapse video microscopy to evaluate how cells cope with
asbestos fibers.
RAW264.7 cells, MeT-5 A and HeLa cells engulfed asbestos fibers,
which
reached not only cytoplasm but also the nucleus. Third, we utilized
supercoiled plasmid DNA to evaluate the ability of each asbestos to
induce DNA double strand breaks (DSB). Crocidolite and amosite, but
not chrysotile, induced DNA DSB in the presence of iron chelators.
We cloned the fragments to identify break sites. DSB occurred
preferentially within repeat sequences and between two G:C sequences.
Finally, i.p. administration of each asbestos to rats induced not
only
formation of nuclear 8-hydroxy-2'-deoxyguanosine in the mesothelia,
spleen, liver and kidney but also significant iron deposits in the
spleen.
Together with the established carcinogenicity of i.p. chrysotile, our
data suggest that asbestos-associated catalytic iron, whether
constitutional or induced by other mechanisms, plays an important
role
in asbestos-induced carcinogenesis and that chemoprevention may be
possible through targeting the catalytic iron.
Cancer science [Cancer Sci]
---------------------------------------------------------------------------*-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

We note you are unable/unwilling to answerthe below, consider again.

Are you sure you want to post this in support of the iron causes all
disease because people eat meat idea?

Iron chelators prevent the cancer causing effects of asbestos ..
"
Now you may have a different understanding .. ?"

No, just what it says, read this part again:

"crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction.""

Note in particular what caused a reaction to be "enhanced" and what
caused no reaction difference. Were they the same kind of thing and
does it contridict your belief above?

Let me quote .. "suppressed by desferal" ..

Desferal is an iron chelator ..

Let me quoate, one chelator "enhances" the bad reaction and 4 others
have no effect. The one that did have a good outcome was not only
because it was a chelator of iron because another increased the bad and
others did nothing. Note also it only happened in the presence of a
chemical. Please tell us what that chemical is. Could that chemical be
the source of problems and could the one chelator that worked act on it
and not the iron because one iron chelator made it worse and others did
nothing?

Characteristics and modifying factors of asbestos-induced oxidative
DNA damage.
Jiang L, Nagai H, Ohara H, Hara S, Tachibana M, Hirano S, Shinohara
Y,
Kohyama N, Akatsuka S, Toyokuni S
Cancer Sci 2008 Sep 4.

Respiratory exposure to asbestos has been linked with mesothelioma in
humans.
However, its carcinogenic mechanism is still unclear.
Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches.
Electron spin resonance analyses revealed that crocidolite and
amosite
containing high amounts of iron, but not chrysotile, catalyzed
hydroxyl radical generation in the presence of H(2)O(2), which was
enhanced by an iron chelator, nitrilotriacetic acid, and suppressed
by
desferal.
Natural iron chelators, such as citrate, adenosine 5'-triphosphate
and
guanosine 5'-triphosphate, did not inhibit this reaction. Second, we
used time-lapse video microscopy to evaluate how cells cope with
asbestos fibers.
RAW264.7 cells, MeT-5 A and HeLa cells engulfed asbestos fibers,
which
reached not only cytoplasm but also the nucleus. Third, we utilized
supercoiled plasmid DNA to evaluate the ability of each asbestos to
induce DNA double strand breaks (DSB). Crocidolite and amosite, but
not chrysotile, induced DNA DSB in the presence of iron chelators.
We cloned the fragments to identify break sites. DSB occurred
preferentially within repeat sequences and between two G:C sequences.
Finally, i.p. administration of each asbestos to rats induced not
only
formation of nuclear 8-hydroxy-2'-deoxyguanosine in the mesothelia,
spleen, liver and kidney but also significant iron deposits in the
spleen.
Together with the established carcinogenicity of i.p. chrysotile, our
data suggest that asbestos-associated catalytic iron, whether
constitutional or induced by other mechanisms, plays an important
role
in asbestos-induced carcinogenesis and that chemoprevention may be
possible through targeting the catalytic iron.
Cancer science [Cancer Sci]
---------------------------------------------------------------------------**-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

We note you are unable/unwilling to answerthe below, consider again.

Are you sure you want to post this in support of the iron causes all
disease because people eat meat idea?

Iron chelators prevent the cancer causing effects of asbestos ..
"
Now you may have a different understanding .. ?"

No, just what it says, read this part again:

"crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction.""

Note in particular what caused a reaction to be "enhanced" and what
caused no reaction difference. Were they the same kind of thing and
does it contridict your belief above?

Let me quote .. "suppressed by desferal" ..

Desferal is an iron chelator ..

Let me quoate, one chelator "enhances" the bad reaction and 4 others
have no effect. The one that did have a good outcome was not only
because it was a chelator of iron because another increased the bad and
others did nothing. Note also it only happened in the presence of a
chemical. Please tell us what that chemical is. Could that chemical be
the source of problems and could the one chelator that worked act on it
and not the iron because one iron chelator made it worse and others did
nothing?

Characteristics and modifying factors of asbestos-induced oxidative
DNA damage.
Jiang L, Nagai H, Ohara H, Hara S, Tachibana M, Hirano S, Shinohara
Y,
Kohyama N, Akatsuka S, Toyokuni S
Cancer Sci 2008 Sep 4.

Respiratory exposure to asbestos has been linked with mesothelioma in
humans.
However, its carcinogenic mechanism is still unclear.
Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches.
Electron spin resonance analyses revealed that crocidolite and
amosite
containing high amounts of iron, but not chrysotile, catalyzed
hydroxyl radical generation in the presence of H(2)O(2), which was
enhanced by an iron chelator, nitrilotriacetic acid, and suppressed
by
desferal.
Natural iron chelators, such as citrate, adenosine 5'-triphosphate
and
guanosine 5'-triphosphate, did not inhibit this reaction. Second, we
used time-lapse video microscopy to evaluate how cells cope with
asbestos fibers.
RAW264.7 cells, MeT-5 A and HeLa cells engulfed asbestos fibers,
which
reached not only cytoplasm but also the nucleus. Third, we utilized
supercoiled plasmid DNA to evaluate the ability of each asbestos to
induce DNA double strand breaks (DSB). Crocidolite and amosite, but
not chrysotile, induced DNA DSB in the presence of iron chelators.
We cloned the fragments to identify break sites. DSB occurred
preferentially within repeat sequences and between two G:C sequences.
Finally, i.p. administration of each asbestos to rats induced not
only
formation of nuclear 8-hydroxy-2'-deoxyguanosine in the mesothelia,
spleen, liver and kidney but also significant iron deposits in the
spleen.
Together with the established carcinogenicity of i.p. chrysotile, our
data suggest that asbestos-associated catalytic iron, whether
constitutional or induced by other mechanisms, plays an important
role
in asbestos-induced carcinogenesis and that chemoprevention may be
possible through targeting the catalytic iron.
Cancer science [Cancer Sci]
---------------------------------------------------------------------------***-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

We note you are unable/unwilling to answerthe below, consider again.

Are you sure you want to post this in support of the iron causes all
disease because people eat meat idea?

Iron chelators prevent the cancer causing effects of asbestos ..
"
Now you may have a different understanding .. ?"

No, just what it says, read this part again:

"crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction.""

Note in particular what caused a reaction to be "enhanced" and what
caused no reaction difference. Were they the same kind of thing and
does it contridict your belief above?

Let me quote .. "suppressed by desferal" ..

Desferal is an iron chelator ..

Let me quoate, one chelator "enhances" the bad reaction and 4 others
have no effect. The one that did have a good outcome was not only
because it was a chelator of iron because another increased the bad and
others did nothing. Note also it only happened in the presence of a
chemical. Please tell us what that chemical is. Could that chemical be
the source of problems and could the one chelator that worked act on it
and not the iron because one iron chelator made it worse and others did
nothing?

Characteristics and modifying factors of asbestos-induced oxidative
DNA damage.
Jiang L, Nagai H, Ohara H, Hara S, Tachibana M, Hirano S, Shinohara
Y,
Kohyama N, Akatsuka S, Toyokuni S
Cancer Sci 2008 Sep 4.

Respiratory exposure to asbestos has been linked with mesothelioma in
humans.
However, its carcinogenic mechanism is still unclear.
Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches.
Electron spin resonance analyses revealed that crocidolite and
amosite
containing high amounts of iron, but not chrysotile, catalyzed
hydroxyl radical generation in the presence of H(2)O(2), which was
enhanced by an iron chelator, nitrilotriacetic acid, and suppressed
by
desferal.
Natural iron chelators, such as citrate, adenosine 5'-triphosphate
and
guanosine 5'-triphosphate, did not inhibit this reaction. Second, we
used time-lapse video microscopy to evaluate how cells cope with
asbestos fibers.
RAW264.7 cells, MeT-5 A and HeLa cells engulfed asbestos fibers,
which
reached not only cytoplasm but also the nucleus. Third, we utilized
supercoiled plasmid DNA to evaluate the ability of each asbestos to
induce DNA double strand breaks (DSB). Crocidolite and amosite, but
not chrysotile, induced DNA DSB in the presence of iron chelators.
We cloned the fragments to identify break sites. DSB occurred
preferentially within repeat sequences and between two G:C sequences.
Finally, i.p. administration of each asbestos to rats induced not
only
formation of nuclear 8-hydroxy-2'-deoxyguanosine in the mesothelia,
spleen, liver and kidney but also significant iron deposits in the
spleen.
Together with the established carcinogenicity of i.p. chrysotile, our
data suggest that asbestos-associated catalytic iron, whether
constitutional or induced by other mechanisms, plays an important
role
in asbestos-induced carcinogenesis and that chemoprevention may be
possible through targeting the catalytic iron.
Cancer science [Cancer Sci]
---------------------------------------------------------------------------*-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

We note you are unable/unwilling to answerthe below, consider again.

Are you sure you want to post this in support of the iron causes all
disease because people eat meat idea?

Iron chelators prevent the cancer causing effects of asbestos ..
"
Now you may have a different understanding .. ?"

No, just what it says, read this part again:

"crocidolite and amosite containing high amounts of iron, but not
chrysotile, catalyzed hydroxyl radical generation in the presence of
H(2)O(2), which was enhanced by an iron chelator, nitrilotriacetic acid,
and suppressed by desferal. Natural iron chelators, such as citrate,
adenosine 5'-triphosphate and guanosine 5'-triphosphate, did not inhibit
this reaction.""

Note in particular what caused a reaction to be "enhanced" and what
caused no reaction difference. Were they the same kind of thing and
does it contridict your belief above?

Let me quote .. "suppressed by desferal" ..

Desferal is an iron chelator ..

Let me quoate, one chelator "enhances" the bad reaction and 4 others
have no effect. The one that did have a good outcome was not only
because it was a chelator of iron because another increased the bad and
others did nothing. Note also it only happened in the presence of a
chemical. Please tell us what that chemical is. Could that chemical be
the source of problems and could the one chelator that worked act on it
and not the iron because one iron chelator made it worse and others did
nothing?

Characteristics and modifying factors of asbestos-induced oxidative
DNA damage.
Jiang L, Nagai H, Ohara H, Hara S, Tachibana M, Hirano S, Shinohara
Y,
Kohyama N, Akatsuka S, Toyokuni S
Cancer Sci 2008 Sep 4.

Respiratory exposure to asbestos has been linked with mesothelioma in
humans.
However, its carcinogenic mechanism is still unclear.
Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches.
Electron spin resonance analyses revealed that crocidolite and
amosite
containing high amounts of iron, but not chrysotile, catalyzed
hydroxyl radical generation in the presence of H(2)O(2), which was
enhanced by an iron chelator, nitrilotriacetic acid, and suppressed
by
desferal.
Natural iron chelators, such as citrate, adenosine 5'-triphosphate
and
guanosine 5'-triphosphate, did not inhibit this reaction. Second, we
used time-lapse video microscopy to evaluate how cells cope with
asbestos fibers.
RAW264.7 cells, MeT-5 A and HeLa cells engulfed asbestos fibers,
which
reached not only cytoplasm but also the nucleus. Third, we utilized
supercoiled plasmid DNA to evaluate the ability of each asbestos to
induce DNA double strand breaks (DSB). Crocidolite and amosite, but
not chrysotile, induced DNA DSB in the presence of iron chelators.
We cloned the fragments to identify break sites. DSB occurred
preferentially within repeat sequences and between two G:C sequences.
Finally, i.p. administration of each asbestos to rats induced not
only
formation of nuclear 8-hydroxy-2'-deoxyguanosine in the mesothelia,
spleen, liver and kidney but also significant iron deposits in the
spleen.
Together with the established carcinogenicity of i.p. chrysotile, our
data suggest that asbestos-associated catalytic iron, whether
constitutional or induced by other mechanisms, plays an important
role
in asbestos-induced carcinogenesis and that chemoprevention may be
possible through targeting the catalytic iron.
Cancer science [Cancer Sci]
---------------------------------------------------------------------------**-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Characteristics and modifying factors of asbestos-induced oxidative
DNA damage.
Jiang L, Nagai H, Ohara H, Hara S, Tachibana M, Hirano S, Shinohara
Y,
Kohyama N, Akatsuka S, Toyokuni S
Cancer Sci 2008 Sep 4.

Respiratory exposure to asbestos has been linked with mesothelioma in
humans.
However, its carcinogenic mechanism is still unclear.
Here we studied the ability of chrysotile, crocidolite and amosite
fibers to induce oxidative DNA damage and the modifying factors using
four distinct approaches.
Electron spin resonance analyses revealed that crocidolite and
amosite
containing high amounts of iron, but not chrysotile, catalyzed
hydroxyl radical generation in the presence of H(2)O(2), which was
enhanced by an iron chelator, nitrilotriacetic acid, and suppressed
by
desferal.
Natural iron chelators, such as citrate, adenosine 5'-triphosphate
and
guanosine 5'-triphosphate, did not inhibit this reaction. Second, we
used time-lapse video microscopy to evaluate how cells cope with
asbestos fibers.
RAW264.7 cells, MeT-5 A and HeLa cells engulfed asbestos fibers,
which
reached not only cytoplasm but also the nucleus. Third, we utilized
supercoiled plasmid DNA to evaluate the ability of each asbestos to
induce DNA double strand breaks (DSB). Crocidolite and amosite, but
not chrysotile, induced DNA DSB in the presence of iron chelators.
We cloned the fragments to identify break sites. DSB occurred
preferentially within repeat sequences and between two G:C sequences.
Finally, i.p. administration of each asbestos to rats induced not
only
formation of nuclear 8-hydroxy-2'-deoxyguanosine in the mesothelia,
spleen, liver and kidney but also significant iron deposits in the
spleen.
Together with the established carcinogenicity of i.p. chrysotile, our
data suggest that asbestos-associated catalytic iron, whether
constitutional or induced by other mechanisms, plays an important
role
in asbestos-induced carcinogenesis and that chemoprevention may be
possible through targeting the catalytic iron.
Cancer science [Cancer Sci]
---------------------------------------------------------------------------**-----


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

On Sep 12, 7:26*am, ironjustice <teamtan...@hotmail.com> wrote:
asbestos <<

"Phytic acid an iron chelator"

Mol Cell Biochem 2002 May-Jun;234-235(1-2):153-60

Asbestos-induced alveolar epithelial cell apoptosis: role of
mitochondrial dysfunction caused by iron-derived free radicals.

Kamp DW, Panduri V, Weitzman SA, Chandel N
Veterans Administration Chicago Health Care System: Lakeside
Division, Northwestern University Medical School, IL, USA.


Asbestos causes asbestosis and malignancies by mechanisms that are
not
fully understood.
Alveolar epithelial cell (AEC) injury by iron-derived reactive oxygen
species (ROS) is one important mechanism implicated.
We previously showed that iron-catalyzed ROS in part mediate asbestos-
inducedAEC DNA damage and apoptosis.
Mitochondria have a critical role in regulating apoptosis after
exposure to agents causing DNA damage but their role in regulating
asbestos-induced apoptosis is unknown.
To determine whether asbestos causes AEC mitochondrial dysfunction, we
exposed A549 cells to amosite asbestos and assessed mitochondrial
membrane potential changes (delta(psi)m) using a fluorometric
technique involving tetremethylrhodamine ethyl ester (TMRE) and
mitotracker green.
We show that amosite asbestos, but not an inert particulate, titanium
dioxide,reduces delta(psi)m after a 4 h exposure period.
Further, the delta(psi)m after 4 h was inversely proportional to the
levels of
apoptosis noted at 24 h as assessed by nuclear morphology as well as
by DNA
nucleosome formation.
A role for iron-derived ROS was suggested by the finding that phytic
acid, an iron chelator, blocked asbestos-induced reductions in A549
cell delta(psi)m and attenuated apoptosis.
Finally, overexpression of Bcl- xl, an anti-apoptotic protein that
localizes to the mitochondria, prevented asbestos-induced decreases in
A549 cell delta(psi)m after 4 h and diminished apoptosis.
We conclude that asbestos alters AEC mitochondrial function in part by
generating iron-derived ROS, which in turn can result in apoptosis.
This suggests that the mitochondrial death pathway is important in
regulating
pulmonary toxicity from asbestos.


PMID: 12162428, UI: 22152072


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk




> Characteristics and modifying factors of asbestos-induced oxidative
> DNA damage.
> Jiang L, Nagai H, Ohara H, Hara S, Tachibana M, Hirano S, Shinohara
> Y,
> Kohyama N, Akatsuka S, Toyokuni S
> Cancer Sci 2008 Sep 4.
>
> Respiratory exposure to asbestos has been linked with mesothelioma in
> humans.
> However, its carcinogenic mechanism is still unclear.
> Here we studied the ability of chrysotile, crocidolite and amosite
> fibers to induce oxidative DNA damage and the modifying factors using
> four distinct approaches.
> Electron spin resonance analyses revealed that crocidolite and
> amosite
> containing high amounts of iron, but not chrysotile, catalyzed
> hydroxyl radical generation in the presence of H(2)O(2), which was
> enhanced by an iron chelator, nitrilotriacetic acid, and suppressed
> by
> desferal.
> Natural iron chelators, such as citrate, adenosine 5'-triphosphate
> and
> guanosine 5'-triphosphate, did not inhibit this reaction. Second, we
> used time-lapse video microscopy to evaluate how cells cope with
> asbestos fibers.
> RAW264.7 cells, MeT-5 A and HeLa cells engulfed asbestos fibers,
> which
> reached not only cytoplasm but also the nucleus. Third, we utilized
> supercoiled plasmid DNA to evaluate the ability of each asbestos to
> induce DNA double strand breaks (DSB). Crocidolite and amosite, but
> not chrysotile, induced DNA DSB in the presence of iron chelators.
> We cloned the fragments to identify break sites. DSB occurred
> preferentially within repeat sequences and between two G:C sequences.
> Finally, i.p. administration of each asbestos to rats induced not
> only
> formation of nuclear 8-hydroxy-2'-deoxyguanosine in the mesothelia,
> spleen, liver and kidney but also significant iron deposits in the
> spleen.
> Together with the established carcinogenicity of i.p. chrysotile, our
> data suggest that asbestos-associated catalytic iron, whether
> constitutional or induced by other mechanisms, plays an important
> role
> in asbestos-induced carcinogenesis and that chemoprevention may be
> possible through targeting the catalytic iron.
> Cancer science [Cancer Sci]
> ---------------------------------------------------------------------------***-----
>
> Who loves ya.
> Tom
>
> Jesus Was A Vegetarian!http://tinyurl.com/634q5a
>
> Man Is A Herbivore!http://tinyurl.com/4rq595
>
> DEAD PEOPLE WALKINGhttp://tinyurl.com/zk9fk

On Sep 12, 7:26*am, ironjustice <teamtan...@hotmail.com> wrote:
asbestos-induced oxidative DNA damage. <<

"Phytic acid blocks asbestos-induced p53 activation"

That would be my free bisphosphonate again.
http://tinyurl.com/2au4yx


P53 mediates amosite asbestos-induced alveolar epithelial cell
mitochondria-regulated apoptosis.
Apr 2006
Vijayalakshmi Panduri,Sailesh Surapureddi,Saul Soberanes,Sigmund A
Weitzman,Navdeep Chandel,David W Kamp
Asbestos causes pulmonary toxicity in part by generating reactive
oxygen species that cause DNA damage.
We previously showed that the mitochondria-regulated (intrinsic)
death
pathway mediates alveolar epithelial cell (AEC) DNA damage and
apoptosis.
Because p53 regulates the DNA damage response in part by inducing
intrinsic cell death, we determined whether p53-dependent
transcriptional activity mediates asbestos-induced AEC mitochondrial
dysfunction and apoptosis.
We show that inhibitors of p53-dependent transcriptional activation
(pifithrin and type 16-E6 protein) block asbestos-induced AEC
mitochondrial membrane potential change (DeltaPsim), caspase 9
activation, and apoptosis.
We demonstrate that asbestos activates p53 promoter activity, mRNA
levels, protein expression, and Bax and p53 mitochondrial
translocation.
Further, pifithrin, E6, phytic acid, or rho(0)-A549 cells (cells
incapable of mitochondrial reactive oxygen species production) block
asbestos-induced p53 activation.
Finally, we show that asbestos augments p53 expression in cells at
the
bronchoalveolar duct junctions of rat lungs and that phytic acid
prevents this.
These data suggest that p53-dependent transcription pathways mediate
asbestos-induced AEC mitochondria-regulated apoptosis.
This suggests an important interactive effect between p53 and the
mitochondria in the pathogenesis of asbestos-induced pulmonary
toxicity that may have broader implications for our understanding of
pulmonary fibrosis and lung cancer.

Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/634q5a


Man Is A Herbivore!
http://tinyurl.com/4rq595


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

anonymous@nowhere.you.know wrote:

> We note you are unable/unwilling to answerthe below, consider again.

You'll never change his belief system. Many people have tried for over 10
years.
Why don't you quit replying to him _unless_ he posts something very
dangerous, which he does from time to time.
(Or trim out the support newsgroup - most other support newsgroups would
appreciate it as well) But that's more work.
You, he, spammers, lawyers looking for mesothelioma patients and others are
making a mess of alt.support.cancer.
Thank you for your kind consideration of my request.