Phytic Acid In Cancer

This is my free bisphosphonate .. again .. http://tinyurl.com/2au4yx

"Phytic acid decreased proliferation of the cell lines"

Effect of phytic acid and inositol on the proliferation
and apoptosis of cells derived from colorectal carcinoma.
Schröterová L, Hasková P, Rudolf E, Cervinka M
Oncol Rep 2010 Mar; 23(3):787-93.

We characterized the effect of phytic acid (inositol hexaphosphate,
IP6) as a potential adjuvant in treatment of colorectal carcinoma
and evaluated the optimal concentration and treatment time to produce
the maximal therapeutic effect.
There is some evidence that myoinositol (Ins) can potentiate anti-
cancer
effects of IP6.
Therefore, we tested both IP6 and Ins individually and in combination
on
human cell lines HT-29, SW-480 and SW-620 derived from colorectal
carcinoma in different stages of malignancy.
The effect of tested chemicals on the cells was measured using
metabolic
activity assay (WST-1), DNA synthesis assay (BrdU), protein synthesis
assay (Brilliant Blue) and apoptosis (caspase-3 activity).
We tested IP6 and Ins at three concentrations: 0.2, 1 and 5 mM for
24,
48 and 72 h.
The concentrations and incubation periods were chosen according to
low
toxicity of the tested substance that was observed in a long-term
clinical
study.
We found that all employed concentrations of IP6 or IP6/Ins decreased
proliferation of the cell lines, with the maximum decrease being
observed
in HT-29 cells.
Metabolic activity of treated cells differed in response to IP6 and
IP6/Ins
treatment; in HT-29 and SW-620 significant decrease was observed only
at
the highest concentration, whereas in SW-480 cells metabolic activity
was
lower at each concentration except 0.2 and 1 mM IP6 or IP6/Ins in 24-
h
incubation.
The results from protein content assay corresponded to the results
obtained
from WST assay.
In addition, we found maximum increase in caspase-3 activity at
concentration
5 mM IP6 or IP6/Ins in HT-29 cells and with IP6 at concentration of
0.2 mM or
IP6/Ins in SW-480 cells with clear indication of Ins enhancing the
proapoptotic
effect of IP6 in all the cell lines studied.

Oncology reports [Oncol Rep]

--------------------------


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Spamming Canuck Dicksucker

On Feb 8, 9:06*am, Ken <flakey...@aol.com> wrote:snip <<

This phytic acid .. do you know what it .. is .. chemist .. ?

Is it another "compound" in your atheist shteating dgfkng brain .. ?

Giiiiiiit .. you stink up the place ..

American atheist same-sex sexual predator ..

This is my free bisphosphonate .. again .. http://tinyurl.com/2au4yx

"Phytic acid decreased proliferation of the cell lines"


Effect of phytic acid and inositol on the proliferation
and apoptosis of cells derived from colorectal carcinoma.
Schröterová L, Hasková P, Rudolf E, Cervinka M
Oncol Rep 2010 Mar; 23(3):787-93.


We characterized the effect of phytic acid (inositol hexaphosphate,
IP6) as a potential adjuvant in treatment of colorectal carcinoma
and evaluated the optimal concentration and treatment time to produce
the maximal therapeutic effect.
There is some evidence that myoinositol (Ins) can potentiate anti-
cancer
effects of IP6.
Therefore, we tested both IP6 and Ins individually and in combination
on
human cell lines HT-29, SW-480 and SW-620 derived from colorectal
carcinoma in different stages of malignancy.
The effect of tested chemicals on the cells was measured using
metabolic
activity assay (WST-1), DNA synthesis assay (BrdU), protein synthesis
assay (Brilliant Blue) and apoptosis (caspase-3 activity).
We tested IP6 and Ins at three concentrations: 0.2, 1 and 5 mM for
24,
48 and 72 h.
The concentrations and incubation periods were chosen according to
low
toxicity of the tested substance that was observed in a long-term
clinical
study.
We found that all employed concentrations of IP6 or IP6/Ins decreased
proliferation of the cell lines, with the maximum decrease being
observed
in HT-29 cells.
Metabolic activity of treated cells differed in response to IP6 and
IP6/Ins
treatment; in HT-29 and SW-620 significant decrease was observed only
at
the highest concentration, whereas in SW-480 cells metabolic activity
was
lower at each concentration except 0.2 and 1 mM IP6 or IP6/Ins in 24-
h
incubation.
The results from protein content assay corresponded to the results
obtained
from WST assay.
In addition, we found maximum increase in caspase-3 activity at
concentration
5 mM IP6 or IP6/Ins in HT-29 cells and with IP6 at concentration of
0.2 mM or
IP6/Ins in SW-480 cells with clear indication of Ins enhancing the
proapoptotic
effect of IP6 in all the cell lines studied.


Oncology reports [Oncol Rep]


--------------------------


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

a spamming Canuck Dicksucker

"American atheist same-sex sexual predator .."

And when one followes the thread back, it is you saying this about
yourself as it is in direct response to one of your posts.

What gives?

Is there something you want us to know?

This is my free bisphosphonate .. again .. http://tinyurl.com/2au4yx


"Phytic acid decreased proliferation of the cell lines"


Effect of phytic acid and inositol on the proliferation
and apoptosis of cells derived from colorectal carcinoma.
Schröterová L, Hasková P, Rudolf E, Cervinka M
Oncol Rep 2010 Mar; 23(3):787-93.


We characterized the effect of phytic acid (inositol hexaphosphate,
IP6) as a potential adjuvant in treatment of colorectal carcinoma
and evaluated the optimal concentration and treatment time to produce
the maximal therapeutic effect.
There is some evidence that myoinositol (Ins) can potentiate anti-
cancer
effects of IP6.
Therefore, we tested both IP6 and Ins individually and in combination
on
human cell lines HT-29, SW-480 and SW-620 derived from colorectal
carcinoma in different stages of malignancy.
The effect of tested chemicals on the cells was measured using
metabolic
activity assay (WST-1), DNA synthesis assay (BrdU), protein synthesis
assay (Brilliant Blue) and apoptosis (caspase-3 activity).
We tested IP6 and Ins at three concentrations: 0.2, 1 and 5 mM for
24,
48 and 72 h.
The concentrations and incubation periods were chosen according to
low
toxicity of the tested substance that was observed in a long-term
clinical
study.
We found that all employed concentrations of IP6 or IP6/Ins decreased
proliferation of the cell lines, with the maximum decrease being
observed
in HT-29 cells.
Metabolic activity of treated cells differed in response to IP6 and
IP6/Ins
treatment; in HT-29 and SW-620 significant decrease was observed only
at
the highest concentration, whereas in SW-480 cells metabolic activity
was
lower at each concentration except 0.2 and 1 mM IP6 or IP6/Ins in 24-
h
incubation.
The results from protein content assay corresponded to the results
obtained
from WST assay.
In addition, we found maximum increase in caspase-3 activity at
concentration
5 mM IP6 or IP6/Ins in HT-29 cells and with IP6 at concentration of
0.2 mM or
IP6/Ins in SW-480 cells with clear indication of Ins enhancing the
proapoptotic
effect of IP6 in all the cell lines studied.


Oncology reports [Oncol Rep]


--------------------------


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

"This is my free bisphosphonate .. again .. http://tinyurl.com/2au4yx"

Have you ever wondered why it is so easy to punch holes in the eating
meat and its iron is the cause of all disease idea?

I'm pretty sure that is you trying to think ..

Don't do that ..

It makes you look stupid ..

This is my free bisphosphonate .. again .. http://tinyurl.com/2au4yx

"Phytic acid decreased proliferation of the cell lines"


Effect of phytic acid and inositol on the proliferation
and apoptosis of cells derived from colorectal carcinoma.
Schröterová L, Hasková P, Rudolf E, Cervinka M
Oncol Rep 2010 Mar; 23(3):787-93.


We characterized the effect of phytic acid (inositol hexaphosphate,
IP6) as a potential adjuvant in treatment of colorectal carcinoma
and evaluated the optimal concentration and treatment time to produce
the maximal therapeutic effect.
There is some evidence that myoinositol (Ins) can potentiate anti-
cancer
effects of IP6.
Therefore, we tested both IP6 and Ins individually and in combination
on
human cell lines HT-29, SW-480 and SW-620 derived from colorectal
carcinoma in different stages of malignancy.
The effect of tested chemicals on the cells was measured using
metabolic
activity assay (WST-1), DNA synthesis assay (BrdU), protein synthesis
assay (Brilliant Blue) and apoptosis (caspase-3 activity).
We tested IP6 and Ins at three concentrations: 0.2, 1 and 5 mM for
24,
48 and 72 h.
The concentrations and incubation periods were chosen according to
low
toxicity of the tested substance that was observed in a long-term
clinical
study.
We found that all employed concentrations of IP6 or IP6/Ins decreased
proliferation of the cell lines, with the maximum decrease being
observed
in HT-29 cells.
Metabolic activity of treated cells differed in response to IP6 and
IP6/Ins
treatment; in HT-29 and SW-620 significant decrease was observed only
at
the highest concentration, whereas in SW-480 cells metabolic activity
was
lower at each concentration except 0.2 and 1 mM IP6 or IP6/Ins in 24-
h
incubation.
The results from protein content assay corresponded to the results
obtained
from WST assay.
In addition, we found maximum increase in caspase-3 activity at
concentration
5 mM IP6 or IP6/Ins in HT-29 cells and with IP6 at concentration of
0.2 mM or
IP6/Ins in SW-480 cells with clear indication of Ins enhancing the
proapoptotic
effect of IP6 in all the cell lines studied.


Oncology reports [Oncol Rep]


--------------------------


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Spamming Canuck Dicksucker

"Don't do that ..

It makes you look stupid .."

Yah gota love it, makes our day. The post preceeding this one was our
friend mr. justice responding to himself

Check it out, he is talking to himself and giving himself this well
deserved advice.

Predators who need a beating ..

I think I've heard that before ..

Oh yeah .. when men were men ..

This is my free bisphosphonate .. again .. http://tinyurl.com/2au4yx


"Phytic acid decreased proliferation of the cell lines"


Effect of phytic acid and inositol on the proliferation
and apoptosis of cells derived from colorectal carcinoma.
Schröterová L, Hasková P, Rudolf E, Cervinka M
Oncol Rep 2010 Mar; 23(3):787-93.


We characterized the effect of phytic acid (inositol hexaphosphate,
IP6) as a potential adjuvant in treatment of colorectal carcinoma
and evaluated the optimal concentration and treatment time to produce
the maximal therapeutic effect.
There is some evidence that myoinositol (Ins) can potentiate anti-
cancer
effects of IP6.
Therefore, we tested both IP6 and Ins individually and in combination
on
human cell lines HT-29, SW-480 and SW-620 derived from colorectal
carcinoma in different stages of malignancy.
The effect of tested chemicals on the cells was measured using
metabolic
activity assay (WST-1), DNA synthesis assay (BrdU), protein synthesis
assay (Brilliant Blue) and apoptosis (caspase-3 activity).
We tested IP6 and Ins at three concentrations: 0.2, 1 and 5 mM for
24,
48 and 72 h.
The concentrations and incubation periods were chosen according to
low
toxicity of the tested substance that was observed in a long-term
clinical
study.
We found that all employed concentrations of IP6 or IP6/Ins decreased
proliferation of the cell lines, with the maximum decrease being
observed
in HT-29 cells.
Metabolic activity of treated cells differed in response to IP6 and
IP6/Ins
treatment; in HT-29 and SW-620 significant decrease was observed only
at
the highest concentration, whereas in SW-480 cells metabolic activity
was
lower at each concentration except 0.2 and 1 mM IP6 or IP6/Ins in 24-
h
incubation.
The results from protein content assay corresponded to the results
obtained
from WST assay.
In addition, we found maximum increase in caspase-3 activity at
concentration
5 mM IP6 or IP6/Ins in HT-29 cells and with IP6 at concentration of
0.2 mM or
IP6/Ins in SW-480 cells with clear indication of Ins enhancing the
proapoptotic
effect of IP6 in all the cell lines studied.


Oncology reports [Oncol Rep]


--------------------------


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Canuck Dumbass

American atheist same-sex sexual predator ..

This is my free bisphosphonate .. again .. http://tinyurl.com/2au4yx


"Phytic acid decreased proliferation of the cell lines"


Effect of phytic acid and inositol on the proliferation
and apoptosis of cells derived from colorectal carcinoma.
Schröterová L, Hasková P, Rudolf E, Cervinka M
Oncol Rep 2010 Mar; 23(3):787-93.


We characterized the effect of phytic acid (inositol hexaphosphate,
IP6) as a potential adjuvant in treatment of colorectal carcinoma
and evaluated the optimal concentration and treatment time to produce
the maximal therapeutic effect.
There is some evidence that myoinositol (Ins) can potentiate anti-
cancer
effects of IP6.
Therefore, we tested both IP6 and Ins individually and in combination
on
human cell lines HT-29, SW-480 and SW-620 derived from colorectal
carcinoma in different stages of malignancy.
The effect of tested chemicals on the cells was measured using
metabolic
activity assay (WST-1), DNA synthesis assay (BrdU), protein synthesis
assay (Brilliant Blue) and apoptosis (caspase-3 activity).
We tested IP6 and Ins at three concentrations: 0.2, 1 and 5 mM for
24,
48 and 72 h.
The concentrations and incubation periods were chosen according to
low
toxicity of the tested substance that was observed in a long-term
clinical
study.
We found that all employed concentrations of IP6 or IP6/Ins decreased
proliferation of the cell lines, with the maximum decrease being
observed
in HT-29 cells.
Metabolic activity of treated cells differed in response to IP6 and
IP6/Ins
treatment; in HT-29 and SW-620 significant decrease was observed only
at
the highest concentration, whereas in SW-480 cells metabolic activity
was
lower at each concentration except 0.2 and 1 mM IP6 or IP6/Ins in 24-
h
incubation.
The results from protein content assay corresponded to the results
obtained
from WST assay.
In addition, we found maximum increase in caspase-3 activity at
concentration
5 mM IP6 or IP6/Ins in HT-29 cells and with IP6 at concentration of
0.2 mM or
IP6/Ins in SW-480 cells with clear indication of Ins enhancing the
proapoptotic
effect of IP6 in all the cell lines studied.


Oncology reports [Oncol Rep]


--------------------------


Who loves ya.
Tom


Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh


Man Is A Herbivore!
http://tinyurl.com/a3cc3


DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk