Selenium and vitamins A and E improve the efficacy of chemo in colon cancer mouse model

The following 2007 AACR meeting abstract reports that dietary
antioxidants selenium and vitamins A and E improved the efficacy of
cisplatin chemotherapy in colon cancer mouse model:

Abstract Number: 623
Presentation Title: Efficacy of dietary antioxidants combined
with a chemotherapeutic agents on human
colon cancer in a fluorescent orthotopic
mouse model
Presentation Start/End Time: Sunday, Apr 15, 2007, 8:00 AM -12:00 PM
Location: Exhibit Hall, Los Angeles Convention
Center
Poster Section: 26
Poster Board Number: 5
Author Block: Huaiyu Ma, Tapas Das, Suzette Pereira,
Xiao-Ming Li, Zhijian Yang, Shigeo Yagi,
Pradip Mukerji, Robert M. Hoffman.
AntiCancer, Inc, San Diego, CA, Ross
Products Division, Abbott Laboratories,
Columbus, OH

We report here the efficacy of dietary antioxidants in combination
with chemotherapy on tumor growth in the orthotopic COLO-205-green
fluorescent protein (GFP) human colon cancer mouse model. The
orthotopically-transplanted nude mice used for the study were randomly
divided into 5 groups (A-E) after surgical orthotopic implantation
(SOI) of tumor tissue. When the tumor reached 100-150 mm3, the
following diets were given: Diet A (modified AIN-93M mature rodent
diet with 4% fish oil); Diet B (modified AIN-93M which contains added
antioxidants vitamin A, vitamin E, and selenium [control]); Diet C
(Diet A without added antioxidants vitamin A, vitamin E, or selenium);
and Diet D (Diet A with 5 times the amount of added antioxidants
vitamin A, vitamin E, and selenium present in Diet B). Each cage was
provided with a feeding jar and a known quantity of food was supplied
fresh each day. Cisplatin, 7 mg/kg, was administrated
intraperitoneally on day 16 after SOI. Throughout the course of
treatment, noninvasive whole-body imaging, based on the GFP expression
of the tumor, permitted visualization of tumor progression. At
sacrifice, the mean tumor weights showed significant statistical
differences in all of the treated groups compared to the negative
control (no Cisplatin treatment) (p£0.001). The mean tumor weight
showed a significant statistical difference between Diet D combined
with Cisplatin compared to the positive control (Diet B) combined with
Cisplatin (p=0.038). Loss of body weight was observed in all Cisplatin
treated groups within the duration of the experiment. Body weight was
stable in the non-Cisplatin treatment group (negative control). Thus,
we have demonstrated that Diet D is effective against tumor growth in
combination with Cisplatin in the fluorescent mouse model of colon
cancer COLO-205-GFP. Diet D, therefore, has important clinical
potential in appropriate combinations with chemotherapeutic agents.


--
Matti Narkia

On Thu, 19 Apr 2007 02:01:01 +0300, Matti Narkia <mna@mbnet.fi> wrote:

>The following 2007 AACR meeting abstract reports that dietary
>antioxidants selenium and vitamins A and E improved the efficacy of
>cisplatin chemotherapy in colon cancer mouse model:
>
>Abstract Number: 623
>Presentation Title: Efficacy of dietary antioxidants combined
>with a chemotherapeutic agents on human
> colon cancer in a fluorescent orthotopic
> mouse model
>Presentation Start/End Time: Sunday, Apr 15, 2007, 8:00 AM -12:00 PM
>Location: Exhibit Hall, Los Angeles Convention
> Center
>Poster Section: 26
>Poster Board Number: 5
>Author Block: Huaiyu Ma, Tapas Das, Suzette Pereira,
> Xiao-Ming Li, Zhijian Yang, Shigeo Yagi,
> Pradip Mukerji, Robert M. Hoffman.
> AntiCancer, Inc, San Diego, CA, Ross
> Products Division, Abbott Laboratories,
> Columbus, OH
>
>We report here the efficacy of dietary antioxidants in combination
>with chemotherapy on tumor growth in the orthotopic COLO-205-green
>fluorescent protein (GFP) human colon cancer mouse model. The
>orthotopically-transplanted nude mice used for the study were randomly
>divided into 5 groups (A-E) after surgical orthotopic implantation
>(SOI) of tumor tissue. When the tumor reached 100-150 mm3, the
>following diets were given: Diet A (modified AIN-93M mature rodent
>diet with 4% fish oil); Diet B (modified AIN-93M which contains added
>antioxidants vitamin A, vitamin E, and selenium [control]); Diet C
>(Diet A without added antioxidants vitamin A, vitamin E, or selenium);
>and Diet D (Diet A with 5 times the amount of added antioxidants
>vitamin A, vitamin E, and selenium present in Diet B). Each cage was
>provided with a feeding jar and a known quantity of food was supplied
>fresh each day. Cisplatin, 7 mg/kg, was administrated
>intraperitoneally on day 16 after SOI. Throughout the course of
>treatment, noninvasive whole-body imaging, based on the GFP expression
>of the tumor, permitted visualization of tumor progression. At
>sacrifice, the mean tumor weights showed significant statistical
>differences in all of the treated groups compared to the negative
>control (no Cisplatin treatment) (p£0.001). The mean tumor weight
>showed a significant statistical difference between Diet D combined
>with Cisplatin compared to the positive control (Diet B) combined with
>Cisplatin (p=0.038). Loss of body weight was observed in all Cisplatin
>treated groups within the duration of the experiment. Body weight was
>stable in the non-Cisplatin treatment group (negative control). Thus,
>we have demonstrated that Diet D is effective against tumor growth in
>combination with Cisplatin in the fluorescent mouse model of colon
>cancer COLO-205-GFP. Diet D, therefore, has important clinical
>potential in appropriate combinations with chemotherapeutic agents.

I would like to remind readers, that this is just a preliminary animal
study, and that everyone should consult their doctor before starting
to take antioxidants during the chemotherapy. There is a possibility
that large doses of these antioxidants may have adverse interactions
with the chemotherapy. Also this abstract handled only colon cancer's
mouse model and cisplatin, we cannot extrapolate its results to other
animal models of cancer (not to mention human cancers) nor to other
chemotherapeutic agents.


--
Matti Narkia