Isocratic High Performance Liquid Chromatography - Coulometric
Electrochemical Detection of Oxytocin in Biological Samples Using C18
Solid Phase Extraction and Polyclonal Antibody-Based Immunoaffinity
Column Purification
by Dr. Mark A. Kukucka and Dr. Hara P. Misra
Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University
Blacksburg, Virginia 24061-0442
Published in: Journal of Chromatography - Biomedical Applications
ABSTRACT: A rapid, sensitive and very specific high-performance liquid
chromatographic (HPLC) method is described for the reliable and
selective quantitation of oxytocin using culture media supernatants.
The isocractic procedure employs solid phase extraction, antibody-
based immunoaffinity purification and dual channel coulometric
electrochemical detection (ECD). The lower limit of detection for this
octapeptide was 40 picograms (40 fmol) on column. Due to its relative
simplicity, specificity and precision, the HPLC-ECD of oxytocin is an
accurate and attractive alternative to many existing quantitative
methods.
Running Title: HPLC-ECD of Oxytocin Using an Ab Column
Key Words: Oxytocin, HPLC, ECD, SPE, Antibody column
Kukucka, Mark A. and Hara P. Misra. 1994. Determination of oxytocin
in biological samples by isocratic high-performance liquid
chromatography with coulometric detection using C18 solid-phase
extraction and polyclonal antibody-based immunoaffinity column
purification. J Chromatogr B Biomed Appl 653(2):139-145.
Oxytocin (thought to be a female hormone) found in adult Leydig cells using HPLC-ECD and immunoaffinity purification 
Linear Mode
